Silica monolithic capillary columns for hilic separations

0492059 - ÚIACH 2019 RIV HU eng J - Journal Article
Moravcová, Dana - Planeta, Josef
Silica monolithic capillary columns for hilic separations.
Hungarian Journal of Industrial Chemistry. Roč. 46, č. 1 (2018), s. 39-42. ISSN 0133-0276
R&D Projects: GA MV VI20172020069; GA ČR(CZ) GA16-03749S
Institutional support: RVO:68081715
Keywords : silica monolithic columns * nucleotide * capillary chromatography
OECD category: Analytical chemistry

The monolithic capillary columns (0.1 mm x 150 mm) prepared by the acidic hydrolysis of tetramethoxysilane (TMOS) in the presence of polyethylene glycol (PEG) and urea were modified by zwitterionic stationary phases and evaluated under HILIC separation conditions by employing a mixture containing nucleosides and nucleotides. The polymeric layer of zwitterions did not affect the high separation efficiency of the original silica monolith. The prepared zwitterionic columns exhibited high separation efficiencies in a range 61,000-289,000 theoretical plates/m for a 2-methacryloyloxyethylphosphorylcholine-based stationary phase and in a range 59,000-135,000 theoretical plates/m for a [2-(methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl)ammonium hydroxide-based stationary phase under optimal separation conditions. The grafted layer of zwitterions on the silica monolithic surface also significantly improved the separation selectivity to compounds of interest.
Permanent Link: http://hdl.handle.net/11104/0285632