Stable isotope compounds - production, detection, and application

0491815 - MBÚ 2019 RIV US eng J - Journal Article
Zachleder, Vilém - Vítová, Milada - Hlavová, Monika - Moudříková, Š. - Mojzeš, P. - Heumann, H. - Becher, J.R. - Bišová, Kateřina
Stable isotope compounds - production, detection, and application.
Biotechnology Advances. Roč. 36, 3 SI (2018), s. 784-797. ISSN 0734-9750. E-ISSN 1873-1899
R&D Projects: GA ČR(CZ) GA17-06264S
Institutional support: RVO:61388971
Keywords : Algae * Deuterium * Magnesium
OECD category: Microbiology
Impact factor: 12.831, year: 2018

Stable isotopes are used in wide fields of application from natural tracers in biology, geology and archeology through studies of metabolic fluxes to their application as tracers in quantitative proteomics and structural biology. We review the use of stable isotopes of biogenic elements (H, C, N, O, S, Mg, Se) with the emphasis on hydrogen and its heavy isotope deuterium. We will discuss the limitations of enriching various compounds in stable isotopes when produced in living organisms. Finally, we overview methods for measuring stable isotopes, focusing on methods for detection in single cells in situ and their exploitation in modern biotechnologies.
Permanent Link: http://hdl.handle.net/11104/0285434