Application of whey protein isolate in bone regeneration: Effects on growth and osteogenic differentiation of bone-forming cells

0489871 - FGÚ 2019 RIV US eng J - Journal Article
Douglas, T.E.L. - Vandrovcová, Marta - Kročilová, Nikola - Keppler, J. K. - Zárubová, Jana - Skirtach, A. G. - Bačáková, Lucie
Application of whey protein isolate in bone regeneration: Effects on growth and osteogenic differentiation of bone-forming cells.
Journal of Dairy Science. Roč. 101, č. 1 (2018), s. 28-36. ISSN 0022-0302. E-ISSN 1525-3198
R&D Projects: GA MZd(CZ) NV15-33018A
Institutional support: RVO:67985823
Keywords : whey protein isolate * cell proliferation * osteogenic differentiation * adipose-derived stem cell
OECD category: Biomaterials (as related to medical implants, devices, sensors)
Impact factor: 3.082, year: 2018

Recently, milk-derived proteins have attracted attention for applications in the biomedical field such as tissue regeneration. Whey protein isolate (WPI), especially its main component beta-lactoglobulin, can modulate immunity and acts as an antioxidant, antitumor, antiviral, and antibacterial agent. There are very few reports of the application of WPI in tissue engineering, especially in bone tissue engineering. In this study, we tested the influence of different concentrations of WPI on behavior of human osteoblast-like Saos-2 cells, human adipose tissue-derived stem cells (ASC), and human neonatal dermal fibroblasts (FIB ). The positive effect on growth was apparent for Saos-2 cells and FIB but not for ASC. However, the expression of markers characteristic for early osteogenic cell differentiation [type-I collagen (COLT) and alkaline phosphatase (ALP)] as well as ALP activity, increased dose-dependently in ASC. Importantly, Saos-2 cells were able to deposit calcium in the presence of WPI, even in a proliferation medium without other supplements that support osteogenic cell differentiation. The results indicate that, depending on the cell type, WPI can act as an enhancer of cell proliferation and osteogenic differentiation. Therefore, enrichment of biomaterials for bone regeneration with WPI seems a promising approach, especially due to the low cost of WPI.
Permanent Link: http://hdl.handle.net/11104/0284292