Metabarcoding analysis of strongylid nematode diversity in two sympatric primate species

0489268 - ÚBO 2018 RIV GB eng J - Journal Article
Pafčo, B. - Čížková, Dagmar - Kreisinger, Jakub - Hasegawa, H. - Vallo, Peter - Shutt, K. - Todd, A. - Petrželková, Klára Judita - Modrý, D.
Metabarcoding analysis of strongylid nematode diversity in two sympatric primate species.
Scientific Reports. Roč. 8, č. 1 (2018), č. článku 5933. ISSN 2045-2322. E-ISSN 2045-2322
R&D Projects: GA ČR GA15-05180S
Institutional support: RVO:68081766
Keywords : western lowland gorillas * oesophagostomum-bifurcum nematoda * internal transcribed spacer * necator-americanus * gut microbiome * gastrointestinal parasites * epidemiologic implications * ancylostoma-duodenale * nonhuman-primates * small ruminants
OECD category: Veterinary science
Impact factor: 4.011, year: 2018

Strongylid nematodes in large terrestrial herbivores such as great apes, equids, elephants, and humans tend to occur in complex communities. However, identification of all species within strongylid communities using traditional methods based on coproscopy or single nematode amplification and sequencing is virtually impossible. High-throughput sequencing (HTS) technologies provide opportunities to generate large amounts of sequence data and enable analyses of samples containing a mixture of DNA from multiple species/genotypes. We designed and tested an HTS approach for strain-level identification of gastrointestinal strongylids using ITS-2 metabarcoding at the MiSeq Illumina platform in samples from two free-ranging non-human primate species inhabiting the same environment, but differing significantly in their host traits and ecology. Although we observed overlapping of particular haplotypes, overall the studied primate species differed in their strongylid nematode community composition. Using HTS, we revealed hidden diversity in the strongylid nematode communities in non-human primates, more than one haplotype was found in more than 90% of samples and coinfections of more than one putative species occurred in 80% of samples. In conclusion, the HTS approach on strongylid nematodes, preferably using fecal samples, represents a time and cost-efficient way of studying strongylid communities and provides a resolution superior to traditional approaches.
Permanent Link: http://hdl.handle.net/11104/0283711