PLANT HORMONES: METHODS AND PROTOCOLS, 3RD EDITION

0487818 - ÚEB 2018 RIV US eng M - Monography Chapter
Novák, Ondřej - Antoniadi, I. - Ljung, K.
High-Resolution Cell-Type Specific Analysis of Cytokinins in Sorted Root Cell Populations of Arabidopsis thaliana.
Methods in Molecular Biology. In: PLANT HORMONES: METHODS AND PROTOCOLS, 3RD EDITION. Totowa: Humana Press, 2017 - (KleineVehn, J.; Sauer, M.), Roč. 1497 (2017), s. 231-248. ISBN 978-1-4939-6467-3
R&D Projects: GA MŠMT(CZ) LO1204; GA MŠMT LK21306; GA ČR GA14-34792S
Institutional support: RVO:61389030
Keywords : tandem mass-spectrometry * solid-phase extraction * gene-expression map * plant-samples * purification * apex * Cytokinins * Fluorescence-activated cell sorting (FACS) * Solid-phase extraction (SPE) * Liquid chromatography-mass spectrometry (LC-MS) * Roots * Arabidopsis * Protoplasts
OECD category: Cell biology

We describe a method combining fluorescence-activated cell sorting (FACS) with one-step miniaturized isolation and accurate quantification of cytokinins (CKs) using ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) to measure these phytohormones in specific cell types of Arabidopsis thaliana roots. The methodology provides information of unprecedented resolution about spatial distributions of CKs, and thus should facilitate attempts to elucidate regulatory networks involved in root developmental processes.
Permanent Link: http://hdl.handle.net/11104/0282439