Range-wide genetic structure of Arabidopsis halleri (Brassicaceae): glacial persistence in multiple refugia and origin of the Northern Hemisphere disjunction

0487795 - BÚ 2018 RIV GB eng J - Journal Article
Šrámková-Fuxová, G. - Záveská, E. - Kolář, Filip - Lučanová, Magdalena - Španiel, S. - Marhold, K.
Range-wide genetic structure of Arabidopsis halleri (Brassicaceae): glacial persistence in multiple refugia and origin of the Northern Hemisphere disjunction.
Botanical Journal of the Linnean Society. Roč. 185, č. 3 (2017), s. 321-342. ISSN 0024-4074. E-ISSN 1095-8339
Institutional support: RVO:67985939
Keywords : Cruciferae * extra-Mediterranean refugia * DNA sequencing
OECD category: Plant sciences, botany
Impact factor: 3.124, year: 2017

We present a comprehensive assessment of the genetic structure and morphological variation of Arabidopsis halleri, a model species for studying heavy metal tolerance and phytoremediation. Based on extensive sampling of 768 individuals from 82 populations across the entire distribution of the species, genotyping using multiple molecular markers (AFLP, nuclear microsatellites and sequences of single-copy nuclear regions and plastid DNA) and phenotyping by multivariate morphometrics, we aimed to reconstruct the range-wide phylogeography and morphological trait evolution in A. halleri populations. In addition, we address general biogeographical questions related to the origin of the striking Northern Hemisphere disjunction (Europe–East Asia) and glacial survival in extra-Mediterranean refugia in Europe. East Asian (Japanese) populations were genetically distinct and slightly depauperate, but their divergence was at levels comparable to major splits within Europe, rejecting both an ancient (old vicariance) and recent (human-mediated spread) origin of the Northern Hemisphere disjunction. In Europe we detected three major genetic lineages of A. halleri, corresponding well with geography (Western–Central Europe, the Alps and the south-eastern Carpathians + the Balkans). Sequence-based divergence estimates indicated a probable Pleistocene origin of these three lineages. This, together with elevated diversity and rarity within each group, suggests in situ glacial persistence of A. halleri in multiple northern refugia of eastern Central Europe. The extensive morphological variation of European A. halleri populations only partly correlated with genetic structure. Rather, it was driven by local environmental characteristics. This suggests a remarkably plastic response of the species to major environmental gradients, manifested by the parallel origin of a distinct alpine phenotype.
Permanent Link: http://hdl.handle.net/11104/0282422