A novel approach to increase the stability of liposomal containers via in prep coating by poly[N-(2-hydroxypropyl)methacrylamide] with covalently attached cholesterol groups

0486084 - ÚMCH 2019 RIV DE eng J - Journal Article
Zaborova, Olga V. - Filippov, Sergey K. - Chytil, Petr - Kováčik, L. - Ulbrich, Karel - Yaroslavov, A. A. - Etrych, Tomáš
A novel approach to increase the stability of liposomal containers via in prep coating by poly[N-(2-hydroxypropyl)methacrylamide] with covalently attached cholesterol groups.
Macromolecular Chemistry and Physics. Roč. 219, č. 7 (2018), s. 1-5, č. článku 1700508. ISSN 1022-1352. E-ISSN 1521-3935
R&D Projects: GA ČR(CZ) GA15-02986S
Institutional support: RVO:61389013
Keywords : copolymers * enhanced longevity * liposomes
OECD category: Polymer science
Impact factor: 2.622, year: 2018

Highly stable liposomes are developed by coating phosphatidylcholine liposomes with amphiphilic N-(2-hydroxypropyl)methacrylamide copolymer. Two approaches in the preparation of coated liposomes are employed: the copolymer is added during (“in prep”) or after (“ex post”) the liposome formation. The influence of polymer concentration and coating method is evaluated using the cryogenic transmission electron microscopy, dynamic light scattering, and small-angle X-ray scattering techniques. The in prep modification significantly increases, up to four weeks, the stability of liposomes against aggregation and makes the liposomal membrane nonpermeable toward an inorganic salt. Such enhanced longevity is attributed to the different structure of in prep coated liposomal membranes.
Permanent Link: http://hdl.handle.net/11104/0283544