gamma H2AX/53BP1 foci as a potential pre-treatment marker of HNSCC tumors radiosensitivity preliminary methodological study and discussion

0485555 - BFÚ 2018 RIV DE eng J - Journal Article
Falk, Martin - Hořáková, Z. - Svobodová, M. - Masařík, M. - Kopečná, Olga - Gumulec, J. - Raudenská, M. - Depeš, Daniel - Bačíková, Alena - Falková, Iva - Binkova, H.
gamma H2AX/53BP1 foci as a potential pre-treatment marker of HNSCC tumors radiosensitivity preliminary methodological study and discussion.
European Physical Journal D. Roč. 71, č. 9 (2017), č. článku 241. ISSN 1434-6060. E-ISSN 1434-6079
R&D Projects: GA ČR(CZ) GA16-12454S
Institutional support: RVO:68081707
Keywords : squamous-cell carcinoma * cancer-associated fibroblasts
OECD category: Biochemistry and molecular biology
Impact factor: 1.393, year: 2017

In order to improve patients' post-treatment quality of life, a shift from surgery to non-surgical (chemo) radio-treatment is recognized in head and neck oncology. However, about half of HNSCC tumors are resistant to irradiation and an efficient marker of individual tumor radiosensitivity is still missing. We analyzed whether various parameters of DNA double strand break (DSB) repair determined in vitro can predict, prior to clinical treatment initiation, the radiosensitivity of tumors. We compared formation and decrease of gamma H2AX/53BP1 foci in 48 h after irradiating tumor cell primocultures with 2 Gy of gamma-rays. To better understand complex tumor behavior, three different cell type primocultures CD90(-), CD90(+), and a mixed culture of these cells were isolated from 1 clinically radioresistant, 2 radiosensitive, and 4 undetermined HPV-HNSCC tumors and followed separately. While DSB repair was delayed and the number of persisting DSBs increased in the radiosensitive tumors, the results for the radioresistant tumor were similar to cultured normal human skin fibroblasts. Hence, DSB repair kinetics/efficiency may correlate with clinical response to radiotherapy for a subset of HNSCC tumors but the size (and therefore practical relevance) of this subset remains to be determined. The same is true for contribution of different cell type primocultures to tumor radioresistance.
Permanent Link: http://hdl.handle.net/11104/0280520