Identification and Characterization of Phospholipids with Very Long Chain Fatty Acids in Brewer's Yeast

0482844 - MBÚ 2018 RIV US eng J - Journal Article
Řezanka, Tomáš - Kolouchová, I. - Gharwalová, L. - Palyzová, Andrea - Sigler, Karel
Identification and Characterization of Phospholipids with Very Long Chain Fatty Acids in Brewer's Yeast.
Lipids. Roč. 52, č. 12 (2017), s. 1007-1017. ISSN 0024-4201. E-ISSN 1558-9307
R&D Projects: GA ČR(CZ) GA17-00027S
Institutional support: RVO:61388971
Keywords : Yeast * Very long chain fatty acids * Negative electrospray ionization
OECD category: Microbiology
Impact factor: 1.936, year: 2017

Yeast lipids and fatty acids (FA) were analyzed in Saccharomyces pastorianus from seven breweries and in the dietary yeast supplement Pangamin. GC-MS identified more than 30 FA, half of which were very-long chain fatty acids (VLCFA) with hydrocarbon chain lengths of aeyen22 C atoms. Positional isomers omega-9 and omega-7 were identified in FA with C18-C28 even-numbered alkyl chains. The most abundant omega-7 isomer was cis-vaccenic acid. The structure of monounsaturated FA was proved by dimethyl disulfide adducts (position of double bonds and cis geometric configuration) and by GC-MS of pyridyl carbinol esters. Ultra-high performance liquid chromatography-tandem mass spectrometry with negative electrospray ionization identified the phospholipids phosphatidylethanolamine, phosphatidylinositol and phosphatidylcholine, with more than 150 molecular species. Wild-type unmutated brewer's yeast strains conventionally used for the manufacture of food supplements were found to contain VLCFA.
Permanent Link: http://hdl.handle.net/11104/0278238