Number of the records: 1
Size-exclusion chromatography and its optimization for material science
- 1.0481739 - ÚMCH 2018 RIV US eng J - Journal Article
Netopilík, Miloš - Trhlíková, Olga
Size-exclusion chromatography and its optimization for material science.
Journal of Material Sciences & Engineering. Roč. 6, č. 4 (2017), s. 1-5, č. článku 358. ISSN 2169-0022
R&D Projects: GA ČR(CZ) GC17-04258J
Grant - others:OPPK(XE) CZ.2.16/3.1.00/24504; AV ČR(CZ) ASRT-16-02
Program: Bilaterální spolupráce
Institutional support: RVO:61389013
Keywords : separation mechanism * size-exclusion chromatography * band broadening function
OECD category: Polymer science
A theoretical analysis of improving the separation power of size exclusion chromatography by decreasing flowrate is conveyed. The variance of the elution curves is larger than expected form estimated dispersity in molecular weight, Mw/Mn. The shape of the elution curves depends strongly on experimental conditions. When the experimental conditions are approaching those to the ideal separation, judged by statistical properties of the curves, both skew and excess kurtosis of the elution curves increase near the exclusion limit in accord with theoretical prediction. In analyses of polystyrene standards by size exclusion chromatography for polymers of molecular weight about thirty thousand and higher, the longitudinal diffusion is not important even at low flow-rates.
Permanent Link: http://hdl.handle.net/11104/0280730
Number of the records: 1