Karyotypes and Distribution of Tandem Repeat Sequences in Brassica nigra Determined by Fluorescence in situ Hybridization

0480444 - BC 2018 RIV CH eng J - Journal Article
Wang, G. - He, Q. - Macas, Jiří - Novák, Petr - Neumann, Pavel - Meng, D. - Zhao, H. - Guo, N. - Han, S. - Zong, M. - Jin, W. - Liu, F.
Karyotypes and Distribution of Tandem Repeat Sequences in Brassica nigra Determined by Fluorescence in situ Hybridization.
Cytogenetic and Genome Research. Roč. 152, č. 3 (2017), s. 158-165. ISSN 1424-8581. E-ISSN 1424-859X
R&D Projects: GA ČR GBP501/12/G090
Institutional support: RVO:60077344
Keywords : asymmetric somatic hybridization * Fluorescence in situ hybridization * Karyotype * (Peri) centromere
OECD category: Genetics and heredity (medical genetics to be 3)
Impact factor: 1.587, year: 2017

Whole-genome shotgun reads were analyzed to determine the repeat sequence composition in the genome of black mustard, Brassica nigra (L.) Koch. The analysis showed that satellite DNA sequences are very abundant in the black mustard genome. The distribution pattern of 7 new tandem repeats (BnSAT13, BnSAT28, BnSAT68, BnSAT76, BnSAT114, BnSAT180, and BnSAT200) on black mustard chromosomes was visualized using fluorescence in situ hybridization (FISH). The FISH signals of BnSAT13 and BnSAT76 provided useful cytogenetic markers, their position and fluorescence intensity allowed for unambiguous identification of all 8 somatic metaphase chromosomes. A karyotype showing the location and fluorescence intensity of these tandem repeat sequences together with the position of rDNAs and centromeric retrotransposons of Brassica (CRB) was constructed. The establishment of the FISH-based karyotype in B. nigra provides valuable information that can be used in detailed analyses of B. nigra accessions and derived allopolyploid Brassica species containing the B genome. (C) 2017 S. Karger AG, Basel
Permanent Link: http://hdl.handle.net/11104/0276222