Nanoparticles with Embedded Porphyrin Photosensitizers for Photooxidation Reactions and Continuous Oxygen Sensing

0480025 - ÚFCH JH 2018 RIV US eng J - Journal Article
Kubát, Pavel - Henke, P. - Berzediová, V. - Štěpánek, M. - Lang, Kamil - Mosinger, Jiří
Nanoparticles with Embedded Porphyrin Photosensitizers for Photooxidation Reactions and Continuous Oxygen Sensing.
ACS Applied Materials and Interfaces. Roč. 9, č. 41 (2017), s. 36229-36238. ISSN 1944-8244. E-ISSN 1944-8252
R&D Projects: GA ČR(CZ) GA16-15020S
Institutional support: RVO:61388955 ; RVO:61388980
Keywords : photooxidation * polystyrene nanoparticles * porphyrins
OECD category: Physical chemistry; Inorganic and nuclear chemistry (UACH-T)
Impact factor: 8.097, year: 2017

We report the synthesis and characterization of sulfonated polystyrene nanoparticles (average diameter 30 +/- 14 nm) with encapsulated 5,10,15,20-tetraphenylporphyrin or ionically entangled tetracationic 5,10,15,20-tetralcis(N-methylpyridinium-4-yl)-porphyrin, their photooxidation properties, and the application of singlet oxygen-sensitized delayed fluorescence (SODF) in oxygen sensing. Both types of nanoparticles effectively photogenerated singlet oxygen, O-2((1)Delta(g)). The O-2((1)Delta(g)) phosphorescence, transient absorption of the porphyrin triplet states, and SODF signals were monitored using time-resolved spectroscopic techniques. The SODF intensity depended on the concentration of the porphyrin photosensitizer and dissolved oxygen and on the temperature. After an initial period (a few microseconds), the kinetics of the SODF process can be approximated as a monoexponential function, and the apparent SODF lifetimes can be correlated with the oxygen concentration. The oxygen sensing based on SODF allowed measurement of the dissolved oxygen in aqueous media in the broad range of oxygen concentrations (0.2-38 mg L-1). The ability of both types of nanoparticles to photooxidize external substrates was predicted by the SODF measurements and proven by chemical tests. The relative photooxidation efficacy was highest at a low porphyrin concentration, as indicated by the highest fluorescence quantum yield (Phi(F)), and it corresponds with negligible inner filter and self-quenching effects. The photooxidation abilities were sensitive to the influence of temperature on the diffusion and solubility of oxygen in both polystyrene and water media and to the rate constant of the O-2((1)Delta(g)) reaction with a substrate. Due to their efficient photogeneration of cytotoxic O-2((1)Delta(g)) at physiological temperatures and their oxygen sensing via SODF, both types of nanoparticles are promising candidates for biomedical applications.
Permanent Link: http://hdl.handle.net/11104/0275952