First description of Cryptosporidium ubiquitum XIIa subtype family in farmed fur animals

0479127 - BC 2018 RIV DE eng J - Journal Article
Kellnerová, K. - Holubová, Nikola - Jandova, Anna - Vejcik, A. - McEvoy, J. - Sak, Bohumil - Kváč, Martin
First description of Cryptosporidium ubiquitum XIIa subtype family in farmed fur animals.
European Journal of Protistology. Roč. 59, JUN (2017), s. 108-113. ISSN 0932-4739. E-ISSN 1618-0429
R&D Projects: GA ČR GA15-01090S
Institutional support: RVO:60077344
Keywords : Apicomplexa * Chinchillas * Cryptosporidium * gp60 * Foxes * Mink * Nutrias
OECD category: Veterinary science
Impact factor: 2.430, year: 2017

This study investigated the prevalence of Cryptosporidium in farmed fur animals in the Czech Republic and Poland. A total of 480 faecal samples were collected from fur animals, including 300 American mink (Mustela vison), 60 silver foxes (Vulpes vulpes), 50 long-tailed chinchillas (Chinchilla lanigera), and 70 nutrias (Myocastor coypus), at 14 farms. Samples were examined for the presence of Cryptosporidium using microscopy (following aniline-carbol-methyl violet staining) and sequence analysis of PCR amplified products. Three mink and two chinchillas from two different farms tested positive for Cryptosporidium ubiquitum DNA. The presence of C. ubiquitum DNA was not associated with diarrhoea. Subtyping of C. ubiquitum isolates by sequence analysis of the 60-kDa glycoprotein gene showed that isolates belonged to the XIIa subtype family, which was previously restricted to humans and ruminants. This suggests that C. ubiquitum subtype Xlla has a broader host range than previously reported. (C) 2017 Elsevier GmbH. All rights reserved.
Permanent Link: http://hdl.handle.net/11104/0275137