RGB color coded images in scanning electron microscopy of biological surfaces

0478391 - MBÚ 2018 RIV US eng J - Journal Article
Kofroňová, Olga - Benada, Oldřich
RGB color coded images in scanning electron microscopy of biological surfaces.
Acta virologica. Roč. 61, č. 3 (2017), s. 349-352. ISSN 0001-723X. E-ISSN 1336-2305
R&D Projects: GA MŠMT(CZ) LO1509; GA ČR(CZ) GA16-20229S
Institutional support: RVO:61388971
Keywords : Biological surfaces * Color images * Scanning electron microscopy
OECD category: Microbiology
Impact factor: 0.696, year: 2017

We present here a methodological approach for the creation of color images in scanning electron microscopy by processing grayscale images taken simultaneously from at least three different detectors in a scanning electron microscope. The final color images are then produced by merging together those grayscale images in RGB color space. We show the images from non-conductive standard sample together with those obtained from real microbiological samples. The first one represents a microbial biofilm naturally grown on fiber glass filter. The other shows individual Bacillus subtilis cells from batch culture. All the image handling was done in open source image processing software ImageJ or GNU Image Manipulation Program (Gimp) or, alternatively, in proprietary AnalySis 3.2 Pro software processing suite.
Permanent Link: http://hdl.handle.net/11104/0274508