Self-assembled chitosan-alginate polyplex nanoparticles containing temoporfin

0476354 - ÚMCH 2018 RIV DE eng J - Journal Article
Brezaniova, I. - Trousil, Jiří - Černochová, Zulfiya - Král, V. - Hrubý, Martin - Štěpánek, Petr - Šlouf, Miroslav
Self-assembled chitosan-alginate polyplex nanoparticles containing temoporfin.
Colloid and Polymer Science. Roč. 295, č. 8 (2017), s. 1259-1270. ISSN 0303-402X. E-ISSN 1435-1536
R&D Projects: GA MŠMT(CZ) LQ1604; GA MŠMT(CZ) ED1.1.00/02.0109; GA ČR(CZ) GA16-02870S; GA MZd(CZ) NV15-25781A; GA MŠMT(CZ) LO1507
Institutional support: RVO:61389013
Keywords : chitosan * sodium alginate * temoporfin
OECD category: Polymer science
Impact factor: 1.967, year: 2017

The aim of this study was to develop biocompatible polyplex nanoparticles with physicochemical properties suitable for the delivery of photosensitizer temoporfin. We prepared, characterized, and compared the two types of polyplex nanoformulations consisting of sodium alginate in combination with chitosan polymer or chitosan oligomer lactate. We obtained the polyplex system by multiple electrostatic interactions between cationic chitosan and anionic alginate and identified key process parameters. Particle size distribution, dispersity, and zeta potential were determined by dynamic light scattering (DLS), and the diameter and the morphology of the individual particles were visualized by a transmission electron microscopy (TEM). It was found that size distribution of the polyplex nanoparticles depends on the concentrations of chitosan and alginate stock solutions and the order and ratio of addition of stock solutions as well as on the pH of the resulting mixture. It appears that the nanoparticles are homogeneous, although micrographs indicate some (vague, indistinct) core-shell structure. The nanoparticles are stable at pH 7.4 (pH of blood plasma) and show only very little drug leak in experiment modeling conditions of blood pool transport to target tissues.
Permanent Link: http://hdl.handle.net/11104/0273176