Cyclosporine A loaded electrospun poly(D,L-lactic acid)/poly(ethylene glycol) nanofibers: drug carriers utilizable in local immunosuppression

0474919 - ÚMCH 2018 RIV US eng J - Journal Article
Širc, Jakub - Hampejsová, Z. - Trnovská, J. - Kozlík, P. - Hrib, Jakub - Hobzová, Radka - Zajícová, Alena - Holáň, Vladimír - Bosáková, Z.
Cyclosporine A loaded electrospun poly(D,L-lactic acid)/poly(ethylene glycol) nanofibers: drug carriers utilizable in local immunosuppression.
Pharmaceutical Research. Roč. 34, č. 7 (2017), s. 1391-1401. ISSN 0724-8741. E-ISSN 1573-904X
R&D Projects: GA ČR(CZ) GA16-04863S; GA MŠMT(CZ) LQ1604; GA MŠMT(CZ) ED1.1.00/02.0109
Institutional support: RVO:61389013 ; RVO:68378041
Keywords : cyclosporine A * drug release kinetics * LC-MS/MS
OECD category: Biochemical research methods; Nano-materials (production and properties) (UEM-P)
Impact factor: 3.335, year: 2017

The present study aims to prepare poly(D,L-lactic acid) (PLA) nanofibers loaded by the immunosuppressant cyclosporine A (CsA, 10 wt%). Amphiphilic poly(ethylene glycol)s (PEG) additives were used to modify the hydrophobic drug release kinetics. Four types of CsA-loaded PLA nanofibrous carriers varying in the presence and molecular weight (MW) of PEG (6, 20 and 35 kDa) were prepared by needleless electrospinning. The samples were extracted for 144 h in phosphate buffer saline or tissue culture medium. A newly developed and validated LC-MS/MS method was utilized to quantify the amount of released CsA from the carriers. In vitro cell experiments were used to evaluate biological activity. Nanofibers containing 15 wt% of PEG showed improved drug release characteristics. Significantly higher release rates were achieved in initial part of experiment (24 h). The highest released doses of CsA were obtained from the nanofibers with PEG of the lowest MW (6 kDa). In vitro experiments on ConA-stimulated spleen cells revealed the biological activity of the released CsA for the whole study period of 144 h and nanofibers containing PEG with the lowest MW exhibited the highest impact (inhibition). The addition of PEG of a particular MW enables to control CsA release from PLA nanofibrous carriers. The biological activity of CsA-loaded PLA nanofibers with PEG persists even after 144 h of previous extraction. Prepared materials are promising for local immunosuppression in various medical applications.
Permanent Link: http://hdl.handle.net/11104/0272108