Hyaluronan polymeric micelles for topical drug delivery

0474586 - MBÚ 2018 RIV GB eng J - Journal Article
Šmejkalová, D. - Muthný, T. - Nešporová, K. - Hermannová, M. - Achbergerová, E. - Huerta-Angelesa, G. - Marek Svoboda, M. - Čepa, M. - Machalová, V. - Luptáková, Dominika - Velebný, V.
Hyaluronan polymeric micelles for topical drug delivery.
Carbohydrate Polymers. Roč. 156, JAN 20 (2017), s. 86-96. ISSN 0144-8617. E-ISSN 1879-1344
Institutional support: RVO:61388971
Keywords : Skin penetration * Polymeric micelle * Hyaluronan
OECD category: Biochemistry and molecular biology
Impact factor: 5.158, year: 2017

Nanosized materials offer promising strategy for topical drug delivery due to their enhancing effect on drug percutaneous transport across the stratum corneum barrier. In this work, polymeric micelles made from hydrophobized hyaluronic acid (HA) were probed for skin delivery. Compared to non-polymeric micelle solutions containing similar drug amount, in vitro skin penetration analysis indicated 3 times larger deposition of drug in the epidermis and 6 times larger drug deposition in the dermis after 5 h of topical treatment in Franz diffusion cells. The drug deposition was further increased with prolonged time of topical treatment. Laser confocal microscopy revealed the accumulation of both, the HA forming the vehicle and the payload, in the epidermis and dermis. Although fluorescent labeling of the HA would suggest co-transport of the HA and the drug, loading FRET pair dyes in the micellar core clearly demonstrated gradual micelle disruption with increasing skin depth. Transcellular penetration was the predominant pathway for the loaded drug. The HA polymeric micelles also demonstrated increased bioactivity of loaded compound in vitro and in vivo. In addition, the loaded micelles were found to be stable in cream formulations and thus they have great potential for topical applications for cosmetic and pharmaceutical purposes.
Permanent Link: http://hdl.handle.net/11104/0271606