On board a raft or boat in the retrovirus sea

0471923 - ÚMG 2017 RIV US eng J - Journal Article
Svoboda, Jan
On board a raft or boat in the retrovirus sea.
Proceedings of the National Academy of Sciences of the United States of America. Roč. 113, č. 15 (2016), s. 3927-3931. ISSN 0027-8424. E-ISSN 1091-6490
R&D Projects: GA ČR GA15-22207S
Institutional support: RVO:68378050
Keywords : cell transformation * virus integration * virus rescue * nonpermissiveness to virus infection
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 9.661, year: 2016

This article summarizes the essential steps in understanding the chicken Rous sarcoma virus (RSV) genome association with a nonpermissive rodent host cell genome. This insight was made possible by in-depth study of RSV-transformed rat XC cells, which were called virogenic because they indefinitely carry virus genetic information in the absence of any infectious virus production. However, the virus was rescued by association of XC cells with chicken fibroblasts, allowing cell fusion between both partners. This and additional studies led to the interpretation that the RSV genome gets integrated into the host cell genome as a provirus. Study of additional rodent virogenic cell lines provided evidence that the transcript of oncogene v-src can be transmitted to other retroviruses and produce cell transformation by itself. As discussed in the text, two main questions related to nonpermissiveness to retrovirus infection remain to be solved. The first is changes in the retrovirus envelope gene allowing virus entry into a nonpermissive cell. The second is the nature of the permissive cell functions required by the nonpermissive cell to ensure infectious virus production. Both lines of investigation are being pursued.
Permanent Link: http://hdl.handle.net/11104/0269288