The interplay between biological and physical scenarios of bacterial death induced by non-thermal plasma

0470525 - FZÚ 2017 RIV GB eng J - Journal Article
Lunov, Oleg - Zablotskyy, Vitaliy A. - Churpita, Olexandr - Jäger, Aleš - Polívka, Leoš - Syková, E. - Dejneka, Alexandr - Kubinová, Šárka
The interplay between biological and physical scenarios of bacterial death induced by non-thermal plasma.
Biomaterials. Roč. 82, Mar (2016), s. 71-83. ISSN 0142-9612. E-ISSN 1878-5905
R&D Projects: GA MŠMT LO1409; GA AV ČR(CZ) Fellowship J. E. Purkyně; GA MŠMT(CZ) LM2011026
Grant - others:FUNBIO(XE) CZ.2.16/3.1.00/21568; AV ČR(CZ) Fellowship J. E. Purkyně
Program: Fellowship J. E. Purkyně
Institutional support: RVO:68378271
Keywords : non-thermal plasma * bacteria * cytotoxicity * apoptosis * bacterial inactivation * reactive oxygen species (ROS)
Subject RIV: BO - Biophysics
Impact factor: 8.402, year: 2016

Direct interactions of plasma matter with living cells and tissues can dramatically affect their functionality, initiating many important effects from cancer elimination to bacteria deactivation. However, the physical mechanisms and biochemical pathways underlying the effects of non-thermal plasma on bacteria and cell fate have still not been fully explored. Here, we report on the molecular mechanisms of non-thermal plasma-induced bacteria inactivation in both Gram-positive and Gram-negative strains. We demonstrate that depending on the exposure time plasma induces either direct physical destruction of bacteria or triggers programmed cell death (PCD) that exhibits characteristic features of apoptosis. The interplay between physical disruption and PCD is on the one hand driven by physical plasma parameters, and on the other hand by biological and physical properties of bacteria.
Permanent Link: http://hdl.handle.net/11104/0268132