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Mitochondrial DNA: Methods and Protocols

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    0470330 - FGÚ 2017 RIV US eng M - Monography Chapter
    Zelenka, Jaroslav - Ježek, Petr
    Import of Fluorescent RNA into Mitochondria of Living Cells.
    Mitochondrial DNA: Methods and Protocols. 3. Totowa: Humana Press, 2016 - (McKenzie, M.), s. 175-181. Methods in Molecular Biology, 1351. ISBN 978-1-4939-3039-5
    R&D Projects: GA ČR(CZ) GPP305/12/P388
    Institutional support: RVO:67985823
    Keywords : 5S-rRNA * mitochondrial DNA * nucleic acid import into mitochondria * fluorescent in vivo hybridization of mtDNA * mitochondrial nucleoids
    Subject RIV: EB - Genetics ; Molecular Biology

    Methods of in vivo visualization and manipulation of mitochondrial genetic machinery are limited due to the need to surpass not only the cytoplasmic membrane but also two mitochondrial membranes. Here, we employ the matrix-addressing sequence of mitochondrial ribosomal 5S-rRNA (termed MAM), which is naturally imported into mammalian mitochondria, to construct an import system for in vivo targeting of mitochondrial (mt) DNA or mtRNA, in order to provide fluorescence hybridization of the desired sequences.
    Permanent Link: http://hdl.handle.net/11104/0268004

     
     
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