High throughput toxicity screening and intracellular detection of nanomaterials.

0469950 - ÚEM 2018 RIV US eng J - Journal Article
Collins, A. R. - Annangi, B. - Rubio, L. - Marcos, R. - Dorn, M. - Merker, C. - Estrela-Lopis, I. - Cimpan, M.R. - Ibrahim, M. - Cimpan, E. - Ostermann, M. - Sauter, A. - Yamani, N.E. - Shaposhnikov, S. - Chevillard, S. - Paget, V. - Grall, R. - Delic, J. - Goni-de-Cerio, F. - Suarez-Merino, B. - Fessard, V. - Hogeveen, K.N. - Fjellsbo, L.M. - Pran, E.R. - Brzicová, Táňa - Topinka, Jan - Silva, M.J. - Leite, P.E. - Ribeiro, A.R. - Granjeiro, J.M. - Grafström, R. - Prina-Mello, A. - Dusinská, M.
High throughput toxicity screening and intracellular detection of nanomaterials.
Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology. Roč. 9, č. 1 (2017), e1413. ISSN 1939-5116. E-ISSN 1939-0041
R&D Projects: GA MŠMT(CZ) LD14002
Institutional support: RVO:68378041
Keywords : in-vitro micronucleus * bioelectrical-impedance analysis * bronchial epithelial-cells
OECD category: Public and environmental health
Impact factor: 6.350, year: 2017

With the growing numbers of nanomaterials (NMs), there is a great demand for rapid and reliable ways of testing NM safetypreferably using in vitro approaches, to avoid the ethical dilemmas associated with animal research. Data are needed for developing intelligent testing strategies for risk assessment of NMs, based on grouping and read-across approaches. The adoption of high throughput screening (HTS) and high content analysis (HCA) for NM toxicity testing allows the testing of numerous materials at different concentrations and on different types of cells, reduces the effect of inter-experimental variation, and makes substantial savings in time and cost. HTS/HCA approaches facilitate the classification of key biological indicators of NM-cell interactions. Validation of in vitroHTS tests is required, taking account of relevance to in vivo results. HTS/HCA approaches are needed to assess dose- and time-dependent toxicity, allowing prediction of in vivo adverse effects. Several HTS/HCA methods are being validated and applied for NM testing in the FP7 project NANoREG, including Label-free cellular screening of NM uptake, HCA, High throughput flow cytometry, Impedance-based monitoring, Multiplex analysis of secreted products, and genotoxicity methodsnamely High throughput comet assay, High throughput in vitro micronucleus assay, and H2AX assay. There are several technical challenges with HTS/HCA for NM testing, as toxicity screening needs to be coupled with characterization of NMs in exposure medium prior to the test, possible interference of NMs with HTS/HCA techniques is another concern. Advantages and challenges of HTS/HCA approaches in NM safety are discussed.
Permanent Link: http://hdl.handle.net/11104/0272756