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Oxidative Stress in the Developing Rat Brain due to Production of Reactive Oxygen and Nitrogen Species

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    0466486 - FGÚ 2017 RIV US eng J - Journal Article
    Wilhelm, Jiří - Vytášek, Richard - Uhlík, Jiří - Vajner, Luděk
    Oxidative Stress in the Developing Rat Brain due to Production of Reactive Oxygen and Nitrogen Species.
    Oxidative Medicine and Cellular Longevity. Roč. 2016, č. 2016 (2016), č. článku 5057610. ISSN 1942-0900. E-ISSN 1942-0994
    R&D Projects: GA ČR(CZ) GAP303/11/0298
    Institutional support: RVO:67985823
    Keywords : oxidative stress * developing rat brain * lipid peroxidation
    Subject RIV: ED - Physiology
    Impact factor: 4.593, year: 2016 ; AIS: 1.038, rok: 2016
    DOI: https://doi.org/10.1155/2016/5057610

    Oxidative stress after birth led us to localize reactive oxygen and nitrogen species (RONS) production in the developing rat brain. Brains were assessed a day prenatally and on postnatal days 1, 2, 4, 8, 14, 30, and 60. Oxidation of dihydroethidium detected superoxide; 6-carboxy-2',7'-dichlorodihydrofluorescein diacetate revealed hydrogen peroxide; immunohistochemical proof of nitrotyrosine and carboxyethyllysine detected peroxynitrite formation and lipid peroxidation, respectively. Blue autofluorescence detected protein oxidation. The foetuses showed moderate RONS production, which changed cyclically during further development. The periods and sites of peak production of individual RONS differed, suggesting independent generation. On day 1, neuronal/glial RONS production decreased indicating that increased oxygen concentration after birth did not cause oxidative stress. Dramatic changes in the amount and the sites of RONS production occurred on day 4. Nitrotyrosine detection reached its maximum. Day 14 represented other vast alterations in RONS generation. Superoxide production in arachnoidal membrane reached its peak. From this day on, the internal elastic laminae of blood vessels revealed the blue autofluorescence. The adult animals produced moderate levels of superoxide; all other markers reached their minimum. There was a strong correlation between detection of nitrotyrosine and carboxyethyllysine probably caused by lipid peroxidation initiated with RONS.
    Permanent Link: http://hdl.handle.net/11104/0264768


     
     
Number of the records: 1  

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