Processing a Biological Tissue from Cryo-SEM to Replica

0465204 - ÚPT 2017 RIV US eng J - Journal Article
Vaškovicová, Naděžda - Hrubanová, Kamila - Krzyžánek, Vladislav
Processing a Biological Tissue from Cryo-SEM to Replica.
Microscopy and Microanalysis. Roč. 22, S3 (2016), s. 236-237. ISSN 1431-9276. E-ISSN 1435-8115
R&D Projects: GA MŠMT(CZ) LO1212; GA MŠMT ED0017/01/01
Institutional support: RVO:68081731
Keywords : Cryo-SEM * ACE600
Subject RIV: JA - Electronics ; Optoelectronics, Electrical Engineering
Impact factor: 1.891, year: 2016

Cryo-SEM is a technique suitable for observation of frozen biological samples. The cryo-preparation of tissue fragments without using cryo-protectants can destroy intracellular compartments and cytoplasm. On the other hand the use of cryoprotectants can prevent deeper etching of the medium and thus it often leads to more complicated detection of cell membranes, which are important for orientation in the sample during its observation. This can be managed by shadowing of the fractured structures by platinum and it may help to heighten the structures as is done in production of replicas which can give more detail at higher magnification. Here we report how the replicas can be prepared in the device ACE600 (Leica microsystems) equipped with the cryo-attachement and two guns for metal sputtering and carbon evaporation, and the combination of cryo-SEM and replicas.
Permanent Link: http://hdl.handle.net/11104/0263869