Plant Cytogenetics

0464630 - ÚEB 2017 RIV US eng M - Monography Chapter
Vrána, Jan - Cápal, Petr - Čihalíková, Jarmila - Kubaláková, Marie - Doležel, Jaroslav
Flow Sorting Plant Chromosomes.
Plant Cytogenetics. Vol. 1429. New York: Humana Press, 2016 - (Kianian, S.; Kianian, P.), s. 119-134. Methods and Protocols, Methods in Molecular Biology. ISBN 978-1-4939-3620-5
R&D Projects: GA MŠMT(CZ) LO1204
Institutional support: RVO:61389030
Keywords : Cell cycle synchronization * Chromosome isolation * Cytogenetic stocks
Subject RIV: EB - Genetics ; Molecular Biology

Nuclear genomes of many important plant species are tremendously complicated to map and sequence. The ability to isolate single chromosomes, which represent small units of nuclear genome, is priceless in many areas of plant research including cytogenetics, genomics, and proteomics. Flow cytometry is the only technique which can provide large quantities of pure chromosome fractions suitable for downstream applications including physical mapping, preparation of chromosome-specific BAC libraries, sequencing, and optical mapping. Here, we describe step-by-step procedure of preparation of liquid suspensions of intact mitotic metaphase chromosomes and their flow cytometric analysis and sorting.
Permanent Link: http://hdl.handle.net/11104/0263462