Cold tolerance is unaffected by oxygen availability despite changes in anaerobic metabolism

0463053 - BC 2017 RIV GB eng J - Journal Article
Boardman, L. - Sorensen, J. G. - Košťál, Vladimír - Šimek, Petr - Terblanche, J. S.
Cold tolerance is unaffected by oxygen availability despite changes in anaerobic metabolism.
Scientific Reports. Roč. 6, SEPT 13 (2016), č. článku 32856. ISSN 2045-2322. E-ISSN 2045-2322
R&D Projects: GA ČR GA13-18509S
Institutional support: RVO:60077344
Keywords : hypoxia * cold * insect
Subject RIV: ED - Physiology
Impact factor: 4.259, year: 2016
http://www.nature.com/articles/srep32856

In an insect model system (/Thaumatotibia leucotreta/), we experimentallymanipulated oxygen availability while measuring metabolic rate, critical thermal minimum (CTmin), supercooling point and changes in 43 metabolites. Results showed that altering oxygen availability did not affect (non-lethal) CTmin nor (lethal) supercooling point. Metabolomic profiling revealed the upregulation of anaerobic metabolites and alterations in concentrations of citric acid cycle intermediates during and after chill coma exposure. Hypoxia exacerbated the anaerobic metabolite responses induced by low temperatures. These results suggest that cold tolerance of /T. leucotreta /larvae is not set by oxygen limitation, and that anaerobic metabolism in these larvae may contribute to their ability to survive in necrotic fruit.

Permanent Link: http://hdl.handle.net/11104/0263436