Biocompatible Size-Defined Dendrimer-Albumin Binding Protein Hybrid Materials as a Versatile Platform for Biomedical Applications

0462486 - MBÚ 2017 RIV DE eng J - Journal Article
Malý, J. - Staněk, Ondřej - Frolík, J. - Malý, M. - Ennen, F. - Appelhans, D. - Semerádtová, A. - Wróbel, D. - Stofik, M. - Knapová, T. - Kuchař, Milan - Červenková Šťastná, Lucie - Čermák, Jan - Šebo, Peter - Malý, Petr
Biocompatible Size-Defined Dendrimer-Albumin Binding Protein Hybrid Materials as a Versatile Platform for Biomedical Applications.
Macromolecular Bioscience. Roč. 16, č. 4 (2016), s. 553-566. ISSN 1616-5187. E-ISSN 1616-5195
R&D Projects: GA ČR(CZ) GA15-05903S; GA MZe QJ1210041; GA MŠMT(CZ) ED1.1.00/02.0109
Institutional support: RVO:61388971 ; RVO:86652036 ; RVO:67985858
Keywords : binding domain proteins * biohybrid structures * HSA
Subject RIV: CE - Biochemistry; EB - Genetics ; Molecular Biology (BTO-N)
Impact factor: 3.238, year: 2016

For the design of a biohybrid structure as a ligand-tailored drug delivery system (DDS), it is highly sophisticated to fabricate a DDS based on smoothly controllable conjugation steps. This article reports on the synthesis and the characterization of biohybrid conjugates based on noncovalent conjugation between a multivalent biotinylated and PEGylated poly(amido amine) (PAMAM) dendrimer and a tetrameric streptavidin-small protein binding scaffold. This protein binding scaffold (SA-ABDwt) possesses nM affinity toward human serum albumin (HSA). Thus, well-defined biohybrid structures, finalized by binding of one or two HSA molecules, are available at each conjugation step in a controlled molar ratio.
Permanent Link: http://hdl.handle.net/11104/0261954