Revelation of the IFN alpha, IL-10, IL-8 and IL-1 beta as promising biomarkers reflecting immuno-pathological mechanisms in porcine Huntington's disease model

0462030 - ÚŽFG 2017 RIV NL eng J - Journal Article
Valeková, Ivona - Jarkovská, Karla - Kotrčová, Eva - Bucci, J. - Ellederová, Zdeňka - Juhás, Štefan - Motlík, Jan - Gadher, S. J. - Kovářová, Hana
Revelation of the IFN alpha, IL-10, IL-8 and IL-1 beta as promising biomarkers reflecting immuno-pathological mechanisms in porcine Huntington's disease model.
Journal of Neuroimmunology. Roč. 293, č. 2 (2016), s. 71-81. ISSN 0165-5728. E-ISSN 1872-8421
R&D Projects: GA MŠMT ED2.1.00/03.0124; GA MŠMT(CZ) LO1609
Institutional support: RVO:67985904
Keywords : porcine Huntington´s disease * immune response * cytokines * central nervous system * serum * biomarkers
Subject RIV: FH - Neurology
Impact factor: 2.720, year: 2016

Studies on Huntington's disease (HD) demonstrated altered immune response in HD gene carriers. Using multiplexing immunoassay, we simultaneously investigated seven cytokines in secretomes of microglia and blood monocytes, cerebrospinal fluid (CSF) and serum collected from transgenic HD minipigs at pre-symptomatic disease stage. Decline in IFN alpha and IL-10 was observed in CSF and secretome of microglia whilst elevated IL-8 and IL-1 beta levels were secreted by microglia. Additionally, IL-8 was increased in serum. The proportion of mutant huntingtin in microglia may have causative impact on cytokine production. IFN alpha, IL-10, IL-8 and IL-1 beta represent promising biomarkers reflecting immuno-pathological mechanisms in porcine HD model.
Permanent Link: http://hdl.handle.net/11104/0261556