Autocrine effects of transgenic resistin reduce palmitate and glucose oxidation in brown adipose tissue

0460150 - FGÚ 2017 RIV US eng J - Journal Article
Pravenec, Michal - Mlejnek, Petr - Zídek, Václav - Landa, Vladimír - Šimáková, Miroslava - Šilhavý, Jan - Strnad, Hynek - Eigner, Sebastian - Eigner-Henke, Kateřina - Škop, V. - Malínská, H. - Trnovská, J. - Kazdová, L. - Drahota, Zdeněk - Mráček, Tomáš - Houštěk, Josef
Autocrine effects of transgenic resistin reduce palmitate and glucose oxidation in brown adipose tissue.
Physiological Genomics. Roč. 48, č. 6 (2016), s. 420-427. ISSN 1094-8341. E-ISSN 1531-2267
R&D Projects: GA MŠMT(CZ) LL1204; GA ČR(CZ) GB14-36804G; GA MZd(CZ) NT14325
Institutional support: RVO:67985823 ; RVO:68378050 ; RVO:61389005
Keywords : brown adipose tissue * autocrine * transgenic * spontaneously hypertensive rat
Subject RIV: FB - Endocrinology, Diabetology, Metabolism, Nutrition
Impact factor: 3.044, year: 2016

Resistin has been originally identified as an adipokine that links obesity to insulin resistance in mice. In our previous studies in spontaneously hypertensive rats (SHR) expressing a nonsecreted form of mouse resistin (Retn) transgene specifically in adipose tissue (SHR-Retn), we have observed an increased lipolysis and serum free fatty acids, ectopic fat accumulation in muscles and insulin resistance. Recently, brown adipose tissue (BAT) has been suggested to play an important role in the pathogenesis of metabolic disturbances. In the current study, we have analyzed autocrine effects of transgenic resistin on BAT glucose and lipid metabolism and mitochondrial function in the SHR-Retn versus nontransgenic SHR controls. We observed that interscapular BAT isolated from SHR-Retn transgenic rats when compared to SHR controls showed a lower relative weight, significantly reduced both basal and insulin stimulated incorporation of palmitate into BAT lipids, and significantly decreased palmitate oxidation and glucose oxidation. Gene expression profiles in BAT identified differentially expressed genes involved in skeletal muscle and connective tissue development, inflammation and MAPK and insulin signaling. These results provide evidence that autocrine effects of resistin attenuate differentiation and activity of BAT and thus may play a role in the pathogenesis of insulin resistance in the rat.
Permanent Link: http://hdl.handle.net/11104/0260278