Sensing microorganisms in the gut triggers the immune response in Eisenia andrei earthworms

0460105 - MBÚ 2017 RIV GB eng J - Journal Article
Dvořák, Jiří - Roubalová, Radka - Procházková, Petra - Rossmann, Pavel - Škanta, František - Bilej, Martin
Sensing microorganisms in the gut triggers the immune response in Eisenia andrei earthworms.
Developmental and Comparative Immunology. Roč. 57, APR 2016 (2016), s. 67-74. ISSN 0145-305X. E-ISSN 1879-0089
R&D Projects: GA MŠMT(CZ) ED1.1.00/02.0109
Institutional support: RVO:61388971
Keywords : Earthworm * Mucosal immunity * CCF
Subject RIV: EE - Microbiology, Virology
Impact factor: 3.218, year: 2016

The tube-within-tube body plan of earthworms is appropriate for studying the interactions of microorganisms with the immune system of body cavities such as the digestive tract and coelom. This study aims to describe the immune response on the molecular and cellular level in the coelomic cavity and the gut of the earthworm Eisenia andrei after experimental microbial challenge by administering two bacterial strains (Escherichia coli and Bacillus subtilis) or yeast Saccharomyces cerevisiae to the environment. The changes in mRNA levels of defense molecules (pattern recognition receptor CCF, lysozyme, fetidin/lysenins) in the coelomocytes and gut tissue were determined by quantitative PCR. The immune response at a cellular level was captured in histological sections, and the expression of CCF was localized using in situ hybridization. Coelomocytes respond to the presence of bacteria in the coelomic cavity by increasing the mRNA levels of defense molecules, especially CCF. The immune response in gut tissue is less affected by microbial stimulation because the epithelial cells of gut exhibit basically strong mRNA synthesis of ccf as a defense against the continuous microbial load in the gut lumen.
Permanent Link: http://hdl.handle.net/11104/0260242