Polycaprolactone foam functionalized with chitosan microparticles – a suitable scaffold for cartilage regeneration.

0459654 - ÚEM 2017 RIV CZ eng J - Journal Article
Filová, Eva - Jakubcová, B. - Danilová, I. - Kuželová Košťáková, E. - Jarošíková, T. - Chernyavskiy, Oleksandr - Hejda, J. - Handl, M. - Beznoska, J. - Nečas, A. - Rosina, J. - Amler, Evžen
Polycaprolactone foam functionalized with chitosan microparticles – a suitable scaffold for cartilage regeneration.
Physiological Research. Roč. 65, č. 1 (2016), s. 121-131. ISSN 0862-8408. E-ISSN 1802-9973
Institutional support: RVO:68378041 ; RVO:67985823
Keywords : poly-epsilon-caprolactone * chitosan * cartilage regeneration * foam
Subject RIV: FI - Traumatology, Orthopedics
Impact factor: 1.461, year: 2016

For biodegradable porous scaffolds to have a potential application in cartilage regeneration, they should enable cell growth and differentiation and should have adequate mechanical properties. In this study, our aim was to prepare biocompatible scaffolds with improved biomechanical properties. To this end, we have developed foam scaffolds from poly-epsilon-caprolactone (PCL) with incorporated chitosan microparticles. The scaffolds were prepared by a salt leaching technique from either 10 or 15 wt% PCL solutions containing 0, 10 and 20 wt% chitosan microparticles, where the same amount and size of NaCl was used as a porogen in all the cases. PCL scaffolds without and with low amounts of chitosan (0 and 10 wt% chitosan) showed higher DNA content than scaffolds with high amounts of chitosan during a 22-day experiment. 10 wt% PCL with 10 and 20 wt% chitosan showed significantly increased viscoelastic properties compared to 15 wt% PCL scaffolds with 0 and 10 wt% chitosan. Thus, 10 wt% PCL scaffolds with 0 wt% and 10 wt% chitosan are potential scaffolds for cartilage regeneration.
Permanent Link: http://hdl.handle.net/11104/0259843