The Supportive Role of Insulin-Like Growth Factor-I in the Differentiation of Murine Mesenchymal Stem Cells into Corneal-Like Cells

0459568 - ÚEM 2017 RIV US eng J - Journal Article
Trošan, Peter - Javorková, Eliška - Zajícová, Alena - Hájková, Michaela - Heřmánková, Barbora - Kössl, Jan - Holáň, Vladimír
The Supportive Role of Insulin-Like Growth Factor-I in the Differentiation of Murine Mesenchymal Stem Cells into Corneal-Like Cells.
Stem Cells and Development. Roč. 7, č. 17 (2016), s. 23156-23169. ISSN 1547-3287. E-ISSN 1557-8534
R&D Projects: GA ČR(CZ) GA14-12580S; GA MŠMT(CZ) ED1.1.00/02.0109; GA MŠMT(CZ) LO1309; GA MŠMT(CZ) LO1508
Institutional support: RVO:68378041
Keywords : mesenchymal stem cells * corneal-like cells * insulin-like growth factor-I * differentiation
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 3.562, year: 2016

Mesenchymal stem cells (MSCs) possess potent immunoregulatory properties and are a source of numerous growth and trophic factors. These properties make them promising candidate for ocular surface regeneration. It was demonstrated that MSCs can express markers of corneal epithelial cells and keratocytes under selected conditions. In previous study, we found that insulin-like growth factor-I (IGF-I) supports the differentiation of limbal stem cells into corneal-like cells. In the present study, we tested differentiation of mouse bone marrow-derived MSCs into corneal epithelial cells using the extract from the cornea and IGF-I. The results show that MSCs cultured in the presence of corneal extract and IGF-I efficiently differentiate into corneal-like cells. The differentiated cells possess characteristics of corneal epithelial cells and keratocytes and at the same time maintain MSC properties.
Permanent Link: http://hdl.handle.net/11104/0259756