Alternative Glycerol Balance Strategies among Saccharomyces Species in Response to Winemaking Stress

0459055 - FGÚ 2017 RIV CH eng J - Journal Article
Pérez-Torrado, R. - Oliveira, B. M. - Zemančíková, Jana - Sychrová, Hana - Querol, A.
Alternative Glycerol Balance Strategies among Saccharomyces Species in Response to Winemaking Stress.
Frontiers in Microbiology. Roč. 7, Mar 31 (2016), s. 435. ISSN 1664-302X. E-ISSN 1664-302X
R&D Projects: GA ČR(CZ) GA15-03708S
EU Projects: European Commission(XE) 264717 - CORNUCOPIA
Institutional support: RVO:67985823
Keywords : Saccharomyces * stress tolerance * glycerol * gene expression
Subject RIV: EE - Microbiology, Virology
Impact factor: 4.076, year: 2016

Production and balance of glycerol is essential for the survival of yeast cells in certain stressful conditions as hyperosmotic or cold shock that occur during industrial processes as winemaking. In this work we have investigated the expression of four genes (GPD1, GPD2, STL1, and FPS1) crucial in the glycerol pool balance in the four species with a biotechnological potential (S. cerevisiae; S. paradoxus; S. uvarum; and S. kudriavzevii), and the ability of strains to grow under osmotic and cold stresses. The results show different pattern and level of expression among the different species, especially for STL1. All our data point to different strategies to handle glycerol accumulation in response to winemaking stresses as hyperosmotic or cold-hyperosmotic stress in the different species, with variable emphasis in the production, influx, or efflux of glycerol
Permanent Link: http://hdl.handle.net/11104/0259311