Inhibition of non-templated nucleotide addition by DNA polymerases in primer extension using twisted intercalating nucleic acid modified templates

0458681 - ÚOCHB 2017 RIV GB eng J - Journal Article
Güixens-Gallardo, Pedro - Hocek, Michal - Perlíková, Pavla
Inhibition of non-templated nucleotide addition by DNA polymerases in primer extension using twisted intercalating nucleic acid modified templates.
Bioorganic and Medicinal Chemistry Letters. Roč. 26, č. 2 (2016), s. 288-291. ISSN 0960-894X. E-ISSN 1464-3405
R&D Projects: GA ČR GBP206/12/G151
Institutional support: RVO:61388963
Keywords : DNA polymerases * nucleotide addition * primer extension * oligonucleotides * twisted intercalating nucleic acid
Subject RIV: CC - Organic Chemistry
Impact factor: 2.454, year: 2016

A simple and elegant method for inhibition of non-templated nucleotide addition by DNA polymerases and for following DNA 30-heterogeneity in enzymatic DNA synthesis by primer extension (PEX) is described. When template bearing ortho-twisted intercalating nucleic acid (ortho-TINA) at the 5'-end is used, non-templated nucleotide addition is reduced in both the A-and B-family DNA polymerases (KOD XL, KOD (exo-), Bst 2.0, Therminator, Deep Vent (exo-) and Taq). Formation of a single oligonucleotide product was observed with ortho-TINA modified template and KOD XL, KOD (exo-), Bst 2.0, Deep Vent (exo-) and Taq DNA polymerases. This approach can be applied to the synthesis of both unmodified and base-modified oligonucleotides.
Permanent Link: http://hdl.handle.net/11104/0258930