Voltammetric detection of phytochelatin transported across unmodified and protoplast modified model phospholipid membranes

0453597 - ÚFCH JH 2017 RIV AT eng J - Journal Article
Navrátil, Tomáš - Nováková, Kateřina - Josypčuk, Bohdan - Sokolová, Romana - Šestáková, Ivana
Voltammetric detection of phytochelatin transported across unmodified and protoplast modified model phospholipid membranes.
Monatshefte fur Chemie. Roč. 147, č. 1 (2016), s. 165-171. ISSN 0026-9247. E-ISSN 1434-4475
R&D Projects: GA ČR(CZ) GAP208/12/1645
Grant - others:Rada Programu interní podpory projektů mezinárodní spolupráce AV ČR(CZ) M200401201
Program: M
Institutional support: RVO:61388955
Keywords : Barley protoplasts * electrochemical impendance spectroscopy * mercury electrode
Subject RIV: CG - Electrochemistry
Impact factor: 1.282, year: 2016

Possibility of phytochelatin PC2 transport was
examined using developed electrochemical cell with two
compartments separated by a phospholipid membrane
created in pores of polycarbonate support. At first cell
penetrating peptides (transportan 10 and mastoparan X)
were used to allow the transfer of phytochelatin PC2. The
results demonstrated that transportan 10 is several times
more efficient than mastoparan X for transferring of phytochelatin
across model membrane. Voltammetry with
hanging mercury drop electrode or with solid amalgam
electrodes was applied for detection of transported species.
For determination of PC2, hanging mercury drop electrode
and mercury meniscus-modified silver solid amalgam
electrode were suitable. Modified model phospholipid
membrane was prepared with parts of protoplasts from
barley roots or leaves, and transport of phytochelatin PC2
has been confirmed. Electrochemical impedance spectroscopy
was utilized to monitor formation, stability, and
qualitatively the transporting process across the supported
phospholipid membranes.
Permanent Link: http://hdl.handle.net/11104/0254366