Tick salivary cystatin sialostatin L2 suppresses IFN responses in mouse dendritic cells

0453400 - BC 2016 RIV GB eng J - Journal Article
Lieskovská, Jaroslava - Páleníková, Jana - Širmarová, J. - Elsterová, Jana - Kotsyfakis, Michalis - Chagas, A. C. - Calvo, E. - Růžek, Daniel - Kopecký, Jan
Tick salivary cystatin sialostatin L2 suppresses IFN responses in mouse dendritic cells.
Parasite immunology. Roč. 37, č. 2 (2015), s. 70-78. ISSN 0141-9838. E-ISSN 1365-3024
R&D Projects: GA ČR GAP302/12/2208
Institutional support: RVO:60077344
Keywords : Tick * Dendritic cells * Interferon * Cystatin
Subject RIV: EC - Immunology
Impact factor: 1.917, year: 2015

Type I interferon (IFN), mainly produced by dendritic cells (DCs), is critical in the host defence against tick-transmitted pathogens. Here, we report that salivary cysteine protease inhibitor from the hard tick Ixodes scapularis, sialostatin L2, affects IFN- mediated immune reactions in mouse dendritic cells. Following IFN receptor ligation, the Janus activated kinases/signal transducer and activator of transcription (JAK/STAT) pathway is activated. We show that sialostatin L2 attenuates phosphorylation of STATs in spleen dendritic cells upon addition of recombinant IFN-. LPS-stimulated dendritic cells release IFN- which in turn leads to the induction of IFN-stimulated genes (ISG) through JAK/STAT pathway activation. The induction of two ISG, interferon regulatory factor 7 (IRF-7) and IP-10, was suppressed by sialostatin L2 in LPS-stimulated dendritic cells. Finally, the interference of sialostatin L2 with IFN action led to the enhanced replication of tick-borne encephalitis virus in DC. In summary, we present here that tick salivary cystatin negatively affects IFN- responses which may consequently increase the pathogen load after transmission via tick saliva.
Permanent Link: http://hdl.handle.net/11104/0254236