Gene expression profiling of changes induced by maternal diabetes in the embryonic heart

0453296 - BTÚ 2016 RIV US eng J - Journal Article
Bohuslavová, Romana - Škvorová, Lada - Čerychová, Radka - Pavlínková, Gabriela
Gene expression profiling of changes induced by maternal diabetes in the embryonic heart.
Reproductive Toxicology. Roč. 57, NOV 2015 (2015), s. 147-156. ISSN 0890-6238. E-ISSN 1873-1708
R&D Projects: GA ČR GA301/09/0117; GA MŠMT(CZ) ED1.1.00/02.0109
Institutional research plan: CEZ:AV0Z50520701
Institutional support: RVO:86652036
Keywords : Diabetic embryopathy * Vascular endothelial growth factor A (VEGF-A) * Gene expression * Cardiovascular defects
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 2.850, year: 2015

Cardiovascular defects are one of the most common congenital defects associated with maternal diabetes. Based on whole embryo gene expression microarray analysis, 11 genes were chosen for temporal expression analysis of diabetes-exposed hearts. The majority of the selected genes were deregulated in diabetes-exposed hearts compared to our controls at E13.5, E14.5, and E18.5. We showed increased hypoxia and HIF-1 alpha protein levels in diabetes-exposed hearts at E10.5, which is a critical time point for the induction of developmental defects associated with diabetic embryopathy. Additionally, we found increased cardiac Vegfa levels that might trigger developmental abnormalities associated with diabetic embryopathy. Our results show that maternal diabetes affects the temporal expression pattern of gene encoding molecules involved in heart development and tissue remodelling and that these molecules might affect heart maturation processes and thus, the final outcome of diabetic pregnancies. (C) 2015 Elsevier Inc. All rights reserved.
Permanent Link: http://hdl.handle.net/11104/0254133