The diameter of nanotubes formed on Ti-6Al-4V alloy controls the adhesion and differentiation of Saos-2 cells

0451947 - FGÚ 2016 RIV NZ eng J - Journal Article
Filová, Elena - Fojt, J. - Krýslová, Markéta - Moravec, H. - Joska, L. - Bačáková, Lucie
The diameter of nanotubes formed on Ti-6Al-4V alloy controls the adhesion and differentiation of Saos-2 cells.
International Journal of Nanomedicine. Roč. 10, 20 Nov (2015), s. 7145-7163. E-ISSN 1178-2013
R&D Projects: GA MZd(CZ) NT13297; GA ČR(CZ) GA15-01558S; GA MŠMT(CZ) ED1.1.00/02.0109
Institutional support: RVO:67985823
Keywords : nanostructure * titanium nanotubes * cell adhesion * Saos-2 cells * osteogenic differentiation
Subject RIV: EI - Biotechnology ; Bionics
Impact factor: 4.320, year: 2015

Ti-6Al-4V-based nanotubes were prepared on a Ti-6Al-4V surface by anodic oxidation on 10 V, 20 V, and 30 V samples. The 10 V, 20 V, and 30 V samples and a control smooth Ti-6Al-4V sample were evaluated in terms of their chemical composition, diameter distribution, and cellular response. The surfaces of the 10 V, 20 V, and 30 V samples consisted of nanotubes of a relatively wide range of diameters that increased with the voltage. Saos-2 cells had a similar initial adhesion on all nanotube samples to the control Ti-6Al-4V sample, but it was lower than on glass. On day 3, the highest concentrations of both vinculin and talin measured by enzyme-linked immunosorbent assay and intensity of immunofluorescence staining were on 30 V nanotubes. On the other hand, the highest concentrations of ALP, type I collagen, and osteopontin were found on 10 V and 20 V samples. The final cellular densities on 10 V, 20 V, and 30 V samples were higher than on glass. Therefore, the controlled anodization of Ti-6Al-4V seems to be a useful tool for preparing nanostructured materials with desirable biological properties
Permanent Link: http://hdl.handle.net/11104/0253002