Selenium supplementation induces mitochondrial biogenesis in trophoblasts

0450924 - BTÚ 2016 RIV GB eng J - Journal Article
Khera, A. - Dong, L. F. - Holland, O. - Vanderlelie, J. - Pasdar, E.A. - Neužil, Jiří - Perkins, A.V.
Selenium supplementation induces mitochondrial biogenesis in trophoblasts.
Placenta. Roč. 36, č. 8 (2015), s. 363-369. ISSN 0143-4004. E-ISSN 1532-3102
Institutional support: RVO:86652036
Keywords : Selenium * Reactive oxygen species * Mitochondrial biogenesis
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 2.972, year: 2015

Introduction: Placental oxidative stress has been implicated in pregnancy complications and previous work has shown that selenium can protect trophoblast mitochondria from oxidative stress. This report examines mitochondrial function and content in trophoblasts supplemented with selenium. Methods: Swan-71, JEG-3 and BeWo cells and placental tissue were incubated with sodium selenite or selenomethionine. Mitochondrial function was examined in a respirometer. Mitochondrial content was determined using RT-PCR. The levels of the mitochondrial biogenesis markers selenoprotein H, PGC-l alpha and NRF-1 was examined by western blotting. Results: Mitochondrial respiration was significantly enhanced post selenium supplementation in cells and tissues. Selenium supplementation increased mitochondrial content and up-regulated mitochondrial biogenesis mediators in cells.
Permanent Link: http://hdl.handle.net/11104/0252119