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Quantum dot-based immunoprobe for optical and electrochemical detection

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    0450514 - ÚIACH 2016 RIV CZ eng C - Conference Paper (international conference)
    Dvořáková, V. - Čadková, M. - Datinská, Vladimíra - Chałupniak, A. - Korecká, L. - Merkoci, A. - Klepárník, Karel - Foret, František - Bílková, Z.
    Quantum dot-based immunoprobe for optical and electrochemical detection.
    CECE 2015. 12th International Interdisciplinary Meeting on Bioanalysis. Brno: Ústav analytické chemie AV ČR, v. v. i, 2015 - (Foret, F.; Křenková, J.; Drobníková, I.; Klepárník, K.), s. 50-53. ISBN 978-80-904959-3-7.
    [CECE 2015. International Interdisciplinary Meeting on Bioanalysis /12./. Brno (CZ), 21.09.2015-23.09.2015]
    Institutional support: RVO:68081715
    Keywords : quantum dots * conjugation * carbodiimide coupling * IgG molecules
    Subject RIV: CB - Analytical Chemistry, Separation

    Three-stage labeling strategy allows the preparation of specific conjugates composed of required antibodies and quantum dots as an extremely sensitive bio-probe applicable in varied immunoassays with following optical and electrochemical detection. This approach is essentially based on carbodiimide chemistry however each single step of whole procedure is separated and controlled which makes this applied protocol oriented, highly efficient and it enables gain of pure antibody-conjugated quantum dots in ready-to-use condition.
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