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Proton-induced direct and indirect damage of plasmid DNA
- 1.0448614 - FZÚ 2016 RIV DE eng J - Journal Article
Vyšín, Luděk - Pachnerová Brabcová, Kateřina - Štěpán, V. - Moretto-Capelle, P. - Bugler, B. - Legube, G. - Cafarelli, P. - Casta, R. - Champeaux, J. P. - Sence, M. - Vlk, M. - Wagner, Richard - Štursa, Jan - Zach, Václav - Incerti, S. - Juha, Libor - Davídková, Marie
Proton-induced direct and indirect damage of plasmid DNA.
Radiation and Environmental Biophysics. Roč. 54, č. 3 (2015), s. 343-352. ISSN 0301-634X. E-ISSN 1432-2099
R&D Projects: GA ČR GA13-28721S; GA MŠMT LD12008; GA MŠMT LM2011019
Institutional support: RVO:68378271 ; RVO:61389005
Keywords : proton radiation * DNA plasmid * direct and indirect effects * clustered damage * repair enzymes
Subject RIV: BO - Biophysics
Impact factor: 1.923, year: 2015
Clustered DNA damage induced by 10, 20 and 30 MeV protons in pBR322 plasmid DNA was investigated. Besides determination of strand breaks, additional lesions were detected using base excision repair enzymes. The plasmid was irradiated in dry form, where indirect radiation effects were almost fully suppressed, and in water solution containing only minimal residual radical scavenger. Simultaneous irradiation of the plasmid DNA in the dry form and in the solution demonstrated the contribution of the indirect effect as prevalent. The damage composition slightly differed when comparing the results for liquid and dry samples. The obtained data were also subjected to analysis concerning different methodological approaches, particularly the influence of irradiation geometry, models used for calculation of strand break yields and interpretation of the strand breaks detected with the enzymes. It was shown that these parameters strongly affect the results.
Permanent Link: http://hdl.handle.net/11104/0250263
Number of the records: 1