Non-thermal plasma mills bacteria: scanning electron microscopy observations

0448062 - FZÚ 2016 RIV US eng J - Journal Article
Lunov, Oleg - Churpita, Olexandr - Zablotskyy, Vitaliy A. - Deyneka, I.G. - Meshkovskii, I.K. - Jäger, Aleš - Syková, Eva - Kubinová, Šárka - Dejneka, Alexandr
Non-thermal plasma mills bacteria: scanning electron microscopy observations.
Applied Physics Letters. Roč. 106, č. 5 (2015), "053703-1"-"053703-5". ISSN 0003-6951. E-ISSN 1077-3118
R&D Projects: GA MŠMT(CZ) LM2011029; GA MŠMT(CZ) LM2011026; GA MŠMT LO1309
Grant - others:AV ČR(CZ) M100101219; SAFMAT(XE) CZ.2.16/3.1.00/22132
Institutional support: RVO:68378271 ; RVO:68378041
Keywords : non-thermal plasma * plasma medicine * bacteria * cells
Subject RIV: BO - Biophysics
Impact factor: 3.142, year: 2015

Non-thermal plasmas hold great promise for a variety of biomedical applications. To ensure safe clinical application of plasma, a rigorous analysis of plasma-induced effects on cell functions is required. Yet mechanisms of bacteria deactivation by non-thermal plasma remain largely unknown. We therefore analyzed the influence of low-temperature atmospheric plasma on Gram-positive and Gram-negative bacteria. Using scanning electron microscopy, we demonstrate that both Gram-positive and Gram-negative bacteria strains in a minute were completely destroyed by helium plasma. In contrast, mesenchymal stem cells (MSCs) were not affected by the same treatment. Furthermore, histopathological analysis of hematoxylin and eosin–stained rat skin sections from plasma–treated animals did not reveal any abnormalities in comparison to control ones. We discuss possible physical mechanisms leading to the shred of bacteria under non-thermal plasma irradiation.
Permanent Link: http://hdl.handle.net/11104/0249796