Number of the records: 1
High Efficacy but Low Potency of delta-Opioid Receptor-G Protein Coupling in Brij-58-Treated, Low-Density Plasma Membrane Fragments
- 1.0447525 - FGÚ 2016 RIV US eng J - Journal Article
Roubalová, Lenka - Vošahlíková, Miroslava - Brejchová, Jana - Sýkora, Jan - Rudajev, Vladimír - Svoboda, Petr
High Efficacy but Low Potency of delta-Opioid Receptor-G Protein Coupling in Brij-58-Treated, Low-Density Plasma Membrane Fragments.
PLoS ONE. Roč. 10, č. 8 (2015), e0135664. ISSN 1932-6203. E-ISSN 1932-6203
R&D Projects: GA ČR(CZ) GAP207/12/0919
Institutional support: RVO:67985823 ; RVO:61388955
Keywords : delta - opioid receptor * G protein coupling * detergent * efficacy * potency
Subject RIV: CE - Biochemistry; CF - Physical ; Theoretical Chemistry (UFCH-W)
Impact factor: 3.057, year: 2015
HEK293 cells expressing DOR-Gi1alpha fusion protein were treated with non-ionic detergent Brij-58 and fractionated by flotation in sucrose density gradient. In optimum range of detergent concentrations, Brij58-treated, low-density membrane fragments exhibited 2-3-fold higher efficacy of agonist-induced G protein activity than membranes prepared in the absence of detergent. The potency of agonist response was significantly decreased. At high detergent concentrations, the functional coupling between delta-opioid receptors and G proteins was completely diminished
Permanent Link: http://hdl.handle.net/11104/0249343
Number of the records: 1