pI-Control in comparative fluorescence gel electrophoresis (CoFGE) using amphoteric azo dyes

0447122 - ÚIACH 2016 RIV NL eng J - Journal Article
Hanneken, M. - Šlais, Karel - König, S.
pI-Control in comparative fluorescence gel electrophoresis (CoFGE) using amphoteric azo dyes.
EuPA Open Proteomics. Roč. 8, SEP (2015), s. 36-39. ISSN 2212-9685
Institutional support: RVO:68081715
Keywords : comparative fluorescence gel * electrophoresis * protein grid * azo dyes
Subject RIV: CB - Analytical Chemistry, Separation
http://ac.els-cdn.com/S2212968515000094/1-s2.0-S2212968515000094-main.pdf?_tid=7c92fa40-56e6-11e5-b36a-00000aab0f01&acdnat=1441798543_19612c0d7466780944bc4ae22173da92

Gel-to-gel variation is a major problem in two-dimensional (2D) gel electrophoresis. Vertical and horizontal comparative fluorescence gel electrophoresis (v/hCoFGE) were recently introduced to improve the reproducibility of protein spot assignment. There, in a combination of 1D- and 2D-PAGE, protein analytes were overlaid with a reference grid which allowed correcting for variation in the y-direction. However, control of the first dimension, pI (x-direction), was missing so far. Here, we applied amphoteric azo dyes for this purpose completing the CoFGE toolkit.
Permanent Link: http://hdl.handle.net/11104/0249054