The utility of three-dimensional optical projection tomography in nerve injection injury imaging

0446968 - FGÚ 2016 RIV US eng J - Journal Article
Cvetko, E. - Čapek, Martin - Damjanovska, M. - Reina, M. A. - Eržen, I. - Stopar-Pintarič, T.
The utility of three-dimensional optical projection tomography in nerve injection injury imaging.
Anaesthesia. Roč. 70, č. 8 (2015), s. 939-947. ISSN 0003-2409. E-ISSN 1365-2044
R&D Projects: GA ČR(CZ) GA13-12412S; GA MŠMT(CZ) LH13028
Institutional support: RVO:67985823
Keywords : optical projection tomography * 3D nerve visualization * nerve disruption
Subject RIV: EA - Cell Biology
Impact factor: 3.794, year: 2015

The objective of this study was to evaluate the ability of optical projection tomography to discern the nerve microarchitecture and injection injury in three dimensions. Five piglets were studied, whose median and lingual nerves were unilaterally injected post mortem with preset volumes of local anaesthetic, excised and subsequently made transparent with benzyl alcohol benzyl benzoate. Images were captured in three dimensions. The same contralateral nerves were used as controls. Using optical projection tomography, we observed differences between the internal organisation of the median and the lingual nerves, which potentially explain the variations in their susceptibility to injury. This was demonstrated in three dimensions as a disruption to the fascicles in the lingual nerve, and their displacement in the median nerve. This new technology offers potential for studying nerve microarchitecture topography and its tolerance to injection injury
Permanent Link: http://hdl.handle.net/11104/0248965