Activity-Based Protein Profiling of Rhomboid Proteases in Liposomes

0446560 - ÚOCHB 2016 RIV DE eng J - Journal Article
Wolf, E. V. - Seybold, M. - Hadravová, Romana - Stříšovský, Kvido - Verhelst, S. H. L.
Activity-Based Protein Profiling of Rhomboid Proteases in Liposomes.
Chembiochem. Roč. 16, č. 11 (2015), s. 1616-1621. ISSN 1439-4227. E-ISSN 1439-7633
R&D Projects: GA MŠMT(CZ) LK11206; GA MŠMT LO1302
Institutional support: RVO:61388963
Keywords : activity-based protein profiling * chemical probes * inhibitors * intramembrane proteases * liposomes
Subject RIV: CE - Biochemistry
Impact factor: 2.850, year: 2015

Although activity-based protein profiling (ABPP) has been used to study a variety of enzyme classes, its application to intramembrane proteases is still in its infancy. Intramembrane proteolysis is an important biochemical mechanism for activating proteins residing within the membrane in a dormant state. Rhomboid proteases (intramembrane serine proteases) are embedded in the lipid bilayers of membranes and occur in all phylogenetic domains. The study of purified rhomboid proteases has mainly been performed in detergent micelle environments. Here we report on the reconstitution of rhomboids in liposomes. Using ABPP, we have been able to detect active rhomboids in large and giant unilamellar vesicles. We have found that the inhibitor profiles of rhomboids in micelles and liposomes are similar, thus validating previous inhibitor screenings. Moreover, fluorescence microscopy experiments on the liposomes constitute the first steps towards activity-based imaging of rhomboid proteases in membrane environments.
Permanent Link: http://hdl.handle.net/11104/0248619