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pI-Control in comparative fluorescence gel electrophoresis (CoFGE) using amphoteric azo dyes
- 1.0446475 - ÚIACH 2016 RIV DE eng J - Journal Article
Hanneken, M. - Šlais, Karel - König, S.
pI-Control in comparative fluorescence gel electrophoresis (CoFGE) using amphoteric azo dyes.
Data in Brief. Roč. 3, JUN (2015), s. 221-228. ISSN 2352-3409. E-ISSN 2352-3409
Institutional support: RVO:68081715
Keywords : 2D-PAGE * CoFGE * Gel electrophoresis
OECD category: Analytical chemistry
Method of publishing: Open access
http://www.sciencedirect.com/science/article/pii/S2352340915000414
Amphoteric azo dyes were used for internal control of pI values in Comparative two-dimensional Fluorescence Gel Electrophoresis (CoFGE) [1]. The 2D-gel images of separated Escherichia coli proteins as well as those of colored amphoteric dyes separated by isoelectric focussing are presented. The latter were used to correct for variation in the first electrophoretic dimension and further improve protein coordinate assignment in 2D-gel electrophoresis. Data tables are supplied to demonstrate pI-value calibration and the effect on the assignment of protein spot coordinates.
Permanent Link: http://hdl.handle.net/11104/0248455
File Download Size Commentary Version Access 0446475.pdf 4 5.5 MB Publisher’s postprint open-access
Number of the records: 1