A Difference in Fatty Acid Composition of Isocaloric High-Fat Diets Alters Metabolic Flexibility in Male C57BL/6JOlaHsd Mice

0446423 - FGÚ 2016 RIV US eng J - Journal Article
Duivenvoorde, L. P. M. - van Schothorst, E. M. - Swarts, H. M. - Kuda, Ondřej - Steenbergh, E. - Termeulen, S. - Kopecký, Jan - Keijer, J.
A Difference in Fatty Acid Composition of Isocaloric High-Fat Diets Alters Metabolic Flexibility in Male C57BL/6JOlaHsd Mice.
PLoS ONE. Roč. 10, č. 6 (2015), e0128515. ISSN 1932-6203. E-ISSN 1932-6203
R&D Projects: GA MŠMT(CZ) 7E10059
Institutional support: RVO:67985823
Keywords : PUFA * satureted fatty acids * high fat diet * metabolic flexibility * indirect calorimetry
Subject RIV: FB - Endocrinology, Diabetology, Metabolism, Nutrition
Impact factor: 3.057, year: 2015

The aim of this study was to determine the effect of high-fat diets with different fatty acid compositions, but similar n6/n3 ratio (with either predominantly PUFAs (HFpu diet) or predominantly SFAs (HFs diet)), on metabolic flexibility. We found that the HFs diet, compared to the HFpu diet, increased mean adipocyte size, liver damage, and ectopic lipid storage in liver and muscle; although, we did not find differences in body weight, total adiposity, adipose tissue health, serum adipokines, whole body energy balance, or circadian rhythm between HFs and HFpu mice. HFs mice were, furthermore, less flexible in their response to both fasting- re-feeding and OxR, while glucose tolerance was indistinguishable. To conclude, the HFs versus the HFpu diet increased ectopic fat storage, liver damage, and mean adipocyte size and reduced metabolic flexibility in male mice. This study underscores the physiological relevance of indirect calorimetry-based challenge tests
Permanent Link: http://hdl.handle.net/11104/0248421