Biophysical and electrochemical studies of protein-nucleic acid interactions

0446270 - BFÚ 2016 RIV AT eng J - Journal Article
Bowater, R. P. - Cobb, A:M. - Pivoňková, Hana - Havran, Luděk - Fojta, Miroslav
Biophysical and electrochemical studies of protein-nucleic acid interactions.
Monatshefte fur Chemie. Roč. 146, č. 5 (2015), s. 723-739. ISSN 0026-9247. E-ISSN 1434-4475
R&D Projects: GA ČR(CZ) GBP206/12/G151; GA ČR(CZ) GAP301/11/2076
Institutional support: RVO:68081707
Keywords : ISOTHERMAL TITRATION CALORIMETRY * OSMIUM-TETROXIDE COMPLEXES * SURFACE-PLASMON RESONANCE
Subject RIV: BO - Biophysics
Impact factor: 1.131, year: 2015

This review is devoted to biophysical and electrochemical methods used for studying protein-nucleic acid (NA) interactions. The importance of NA structure and protein-NA recognition for essential cellular processes, such as replication or transcription, is discussed to provide background for description of a range of biophysical chemistry methods that are applied to study a wide scope of protein-DNA and protein-RNA complexes. These techniques employ different detection principles with specific advantages and limitations and are often combined as mutually complementary approaches to provide a complete description of the interactions. Electrochemical methods have proven to be of great utility in such studies because they provide sensitive measurements and can be combined with other approaches that facilitate the protein-NA interactions. Recent applications of electrochemical methods in studies of protein-NA interactions are discussed in detail.
Permanent Link: http://hdl.handle.net/11104/0248250