Phosphatidylcholine covalently linked to a methacrylate-based monolith as a biomimetic stationary phase for capillary liquid chromatography

0444787 - ÚIACH 2016 RIV NL eng J - Journal Article
Moravcová, Dana - Carrasco-Correa, E. J. - Planeta, Josef - Lämmerhofer, M. - Wiedmer, S. K.
Phosphatidylcholine covalently linked to a methacrylate-based monolith as a biomimetic stationary phase for capillary liquid chromatography.
Journal of Chromatography A. Roč. 1402, JUL (2015), s. 27-35. ISSN 0021-9673. E-ISSN 1873-3778
R&D Projects: GA MV VG20112015021
Institutional support: RVO:68081715
Keywords : Capillary LC * analyte–membrane interaction * monolithic column * phospholipids
Subject RIV: CB - Analytical Chemistry, Separation
Impact factor: 3.926, year: 2015

Methacrylate-based monoliths (150 × 0.1 mm) were modified by soybean phosphatidylcholine through 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide coupling to obtain stationaryphases suitable to mimic cell surface membranes. The prepared stationary phases were evaluated through LC studies on the retention of low-molar mass model analytes including neutral, acidic, and basic compounds.
Permanent Link: http://hdl.handle.net/11104/0247268