Evaluation of poly(ethylene glycol)-coated monodispersed magnetic poly(2-hydroxyethyl methacrylate) and poly(glycidyl methacrylate) microspheres by PCR

0444691 - ÚMCH 2016 RIV GB eng J - Journal Article
Horák, Daniel - Hlídková, Helena - Trachtová, Š. - Šlouf, Miroslav - Rittich, B. - Španová, A.
Evaluation of poly(ethylene glycol)-coated monodispersed magnetic poly(2-hydroxyethyl methacrylate) and poly(glycidyl methacrylate) microspheres by PCR.
European Polymer Journal. Roč. 68, July (2015), s. 687-696. ISSN 0014-3057. E-ISSN 1873-1945
R&D Projects: GA ČR GAP206/12/0381; GA MŠMT(CZ) LH14318
Institutional support: RVO:61389013
Keywords : magnetic microspheres * poly(ethylene glycol) * real-time PCR
Subject RIV: CD - Macromolecular Chemistry
Impact factor: 3.485, year: 2015

New monodisperse magnetic poly(2-hydroxyethyl methacrylate) and poly(glycidyl methacrylate) microspheres were synthesized, coated with poly(ethylene glycol) and thoroughly characterized using several methods, such as scanning and transmission electron microscopy equipped with energy-dispersive X-ray spectroscopy (EDX), elemental analysis, ATR FT-IR and atomic absorption spectroscopy. The effect of different coatings of the microspheres on the DNA amplification was finally investigated using a real-time polymerase chain reaction (qPCR). The particles with a relatively high density of poly(ethylene glycol) and a low amount of carboxyl groups on the surface appeared suitable for DNA isolation.
Permanent Link: http://hdl.handle.net/11104/0248057